Quantifying substrate uptake by individual cells of marine bacterioplankton by catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography

Author(s)
Eva Sintes, Gerhard J. Herndl
Abstract

Catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography (MICRO-CARD-FISH) is increasingly being used to obtain qualitative information on substrate uptake by individual members of specific prokaryotic communities. Here we evaluated the potential for using this approach quantitatively by relating the measured silver grain area around cells taking up 3H-labeled leucine to bulk leucine uptake measurements. The increase in the silver grain area over time around leucine-assimilating cells of coastal bacterial assemblages was linear during 4 to 6 h of incubation. By establishing standardized conditions for specific activity levels and concomitantly performing uptake measurements with the bulk community, MICRO-CAMD-FISH can be used quantitatively to determine uptake rates on a single-cell level. Therefore, this approach allows comparisons of single-cell activities for bacterial communities obtained from different sites or growing under different ecological conditions.

Organisation(s)
External organisation(s)
Royal Netherlands Institute for Sea Research
Journal
Applied and Environmental Microbiology
Volume
72
Pages
7022-7028
No. of pages
7
ISSN
0099-2240
DOI
https://doi.org/10.1128/AEM.00763-06
Publication date
11-2006
Peer reviewed
Yes
Austrian Fields of Science 2012
106021 Marine biology
ASJC Scopus subject areas
Environmental Science(all), Biotechnology, Microbiology
Sustainable Development Goals
SDG 14 - Life Below Water
Portal url
https://ucrisportal.univie.ac.at/en/publications/quantifying-substrate-uptake-by-individual-cells-of-marine-bacterioplankton-by-catalyzed-reporter-deposition-fluorescence-in-situ-hybridization-combined-with-microautoradiography(d4eb2de8-2ac9-4569-9a63-78cfd48eab11).html