Quantifying substrate uptake by individual cells of marine bacterioplankton by catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography
- Author(s)
- Eva Sintes, Gerhard J. Herndl
- Abstract
Catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography (MICRO-CARD-FISH) is increasingly being used to obtain qualitative information on substrate uptake by individual members of specific prokaryotic communities. Here we evaluated the potential for using this approach quantitatively by relating the measured silver grain area around cells taking up 3H-labeled leucine to bulk leucine uptake measurements. The increase in the silver grain area over time around leucine-assimilating cells of coastal bacterial assemblages was linear during 4 to 6 h of incubation. By establishing standardized conditions for specific activity levels and concomitantly performing uptake measurements with the bulk community, MICRO-CAMD-FISH can be used quantitatively to determine uptake rates on a single-cell level. Therefore, this approach allows comparisons of single-cell activities for bacterial communities obtained from different sites or growing under different ecological conditions.
- Organisation(s)
- External organisation(s)
- Royal Netherlands Institute for Sea Research
- Journal
- Applied and Environmental Microbiology
- Volume
- 72
- Pages
- 7022-7028
- No. of pages
- 7
- ISSN
- 0099-2240
- DOI
- https://doi.org/10.1128/AEM.00763-06
- Publication date
- 11-2006
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106021 Marine biology
- ASJC Scopus subject areas
- Environmental Science(all), Biotechnology, Microbiology
- Sustainable Development Goals
- SDG 14 - Life Below Water
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/quantifying-substrate-uptake-by-individual-cells-of-marine-bacterioplankton-by-catalyzed-reporter-deposition-fluorescence-in-situ-hybridization-combined-with-microautoradiography(d4eb2de8-2ac9-4569-9a63-78cfd48eab11).html