Quantification of aquatic viruses by flow cytometry
- Author(s)
- Corina P. D. Brussaard, Jerome P. Payet, Christian Winter, Markus Weinbauer
- Abstract
For many laboratories, flow cytometry is becoming the routine method for quantifying viruses in aquatic systems
because of its high reproducibility, high sample throughput, and ability to distinguish several subpopulations
of viruses. Comparison of viral counts between flow cytometry and epifluorescence microscopy typically
shows slopes that are statistically not distinguishable from 1, thus confirming the usefulness of flow cytometry.
Here we describe in detail all steps in the procedure, discuss potential problems, and offer solutions.
- Organisation(s)
- Functional and Evolutionary Ecology
- External organisation(s)
- Royal Netherlands Institute for Sea Research, University of British Columbia (UBC), Laboratoire d'Océanographie de Villefranche
- Pages
- 102-109
- No. of pages
- 8
- Publication date
- 2010
- Austrian Fields of Science 2012
- 106022 Microbiology
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/quantification-of-aquatic-viruses-by-flow-cytometry(63a4e5c5-9e80-4172-9d8f-780534ed7d22).html