Viral Communities in the Global Deep Ocean Conveyor Belt Assessed by Targeted Viromics

Author(s)
Daniele De Corte, Joaquin Martinez Martinez, Mariana Silvia Cretoiu, Yoshihiro Takaki, Takuro Nunoura, Eva Sintes, Gerhard J. Herndl, Taichi Yokokawa
Abstract

Viruses are an abundant, diverse and dynamic component of marine and terrestrial ecosystems. In the ocean, viruses play a key role in the biogeochemical cycles and controlling microbial abundance, diversity and evolution. Recent metagenomics studies assessed the structure of the viral community in the upper ocean. However, little is known about the compositional changes in viral communities along the deep ocean conveyor belt. To assess potential changes in the viral community in the global deep-water circulation system, water samples were collected in the core of the North Atlantic Deep Water (NADW) (similar to 2,500 m) and Pacific Antarctic Bottom Water (similar to 4,000 m). Microbial and viral abundance were evaluated by flow cytometry. Subsequently, flow cytometry was used to sort virus-like particles and next generation sequencing was applied to build DNA libraries from the sorted virus populations. The viral communities were highly diverse across different oceanic regions with high dissimilarity between samples. Only 18% of the viral protein clusters were shared between the NADW and the Pacific Antarctic Bottom Water. Few viral groups, mainly associated with uncultured environmental and uncultured Mediterranean viruses were ubiquitously distributed along the global deep-water circulation system. Thus, our results point to a few groups of widely distributed abundant viruses in addition to the presence of rare and diverse types of viruses at a local scale.

Organisation(s)
Department of Limnology and Bio-Oceanography
Journal
Frontiers in Microbiology
Volume
10
No. of pages
14
ISSN
1664-302X
DOI
https://doi.org/10.3389/fmicb.2019.01801
Publication date
08-2019
Publication status
Published
Peer reviewed
Yes
Austrian Fields of Science 2012
106021 Marine biology
Keywords
targeted viromics, deep ocean circulation, viruses, deep ocean, next generation sequencing, DISSOLVED ORGANIC-CARBON, LARGE-SCALE DISTRIBUTION, MARINE VIRUSES, BACTERIOPLANKTON DISTRIBUTION, PACIFIC, WATERS, METAGENOMICS, DIVERSITY, ABUNDANCE, BACTERIA
Portal url
https://ucris.univie.ac.at/portal/en/publications/viral-communities-in-the-global-deep-ocean-conveyor-belt-assessed-by-targeted-viromics(a20dbf8f-7c21-40bd-8108-b98a3d8116ee).html